Giant panda Yuan Yuan enjoys a special birthday cake in Taipei Zoo in Taipei, southeast China's Taiwan, Aug. 30, 2016. (Xinhua/Song Zhenping)
(ECNS) -- Taipei Zoo announced that giant panda Yuan Yuan is not pregnant after receiving artificial insemination in mid-March.
Veterinarians collected sperm from male panda Tuan Tuan and then injected it in Yuan Yuan on March 17.
Efforts to help Yuan Yuan conceive a second cub are now diagnosed as failed after 119 days of observation and checks, according to the zoo.
"An expert review and comprehensive evaluation shows Yuan Yuan is not pregnant and all suspected signs of pregnancy have disappeared gradually. The panda is expected to get back its appetite and mobility soon and meet tourists again," it was added.
Tuan Tuan and Yuan Yuan were given as a goodwill gift to Taiwan by the Chinese mainland in 2008, and are both now about 12 years old. They had their first female cub Yuan Zai on July 6, 2013, also by artificial insemination.